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murine intestinal epithelial iec-6 cells  (BioResource International Inc)

 
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    BioResource International Inc murine intestinal epithelial iec-6 cells
    (A, B) Cell permeability in murine epithelial <t>IEC-6</t> cells following treatment of 5-FU. (A) After 5-FU treatment, permeability of IEC-6 cells increases in a concentration-dependent manner (5 and 10 μM). (B) siRNA-mediated knockdown of SPAK expression in IEC-6 cells blocks this increase in cell permeability compared to control cells treated with 5-FU (10 μM). (C, D) SPAK-knockdown modulates the effects of 5-FU treatment on the proliferation and survival of IEC-6 cells. Control and SPAK-KD IEC-6 cells were treated with 10 µM 5-FU and dimethyl sulfoxide (DMSO) solvent for 3 h, respectively. Untreated and 5-FU-treated control or SPAK-KD IEC-6 cells were analyzed to determine the mean fluorescence index (MFI) of PCNA expression (C) and the percentage of annexin-V-positive cells (D) , respectively. (C) Representative flow cytometry assays are shown in the left panel, and the MFI of PCNA is shown in the right panel. (D) Representative flow cytometry assays (left panel) and the frequencies (right panel) of annexin-V-positive IEC-6 cells are presented. Data corresponding to MFIs or frequencies are representatives of at least three mice in each group, presented as the mean ± SEM. Two-tailed Student’s unpaired t -tests were used for statistical analysis. *** P < 0.0001; ** P < 0.01; * P < 0.05 n.s., not significant.
    Murine Intestinal Epithelial Iec 6 Cells, supplied by BioResource International Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/murine+intestinal+epithelial+iec-6+cells/pmc08649624-43-0-5?v=BioResource+International+Inc
    Average 90 stars, based on 1 article reviews
    murine intestinal epithelial iec-6 cells - by Bioz Stars, 2026-07
    90/100 stars

    Images

    1) Product Images from "SPAK Deficiency Attenuates Chemotherapy-Induced Intestinal Mucositis"

    Article Title: SPAK Deficiency Attenuates Chemotherapy-Induced Intestinal Mucositis

    Journal: Frontiers in Oncology

    doi: 10.3389/fonc.2021.733555

    (A, B) Cell permeability in murine epithelial IEC-6 cells following treatment of 5-FU. (A) After 5-FU treatment, permeability of IEC-6 cells increases in a concentration-dependent manner (5 and 10 μM). (B) siRNA-mediated knockdown of SPAK expression in IEC-6 cells blocks this increase in cell permeability compared to control cells treated with 5-FU (10 μM). (C, D) SPAK-knockdown modulates the effects of 5-FU treatment on the proliferation and survival of IEC-6 cells. Control and SPAK-KD IEC-6 cells were treated with 10 µM 5-FU and dimethyl sulfoxide (DMSO) solvent for 3 h, respectively. Untreated and 5-FU-treated control or SPAK-KD IEC-6 cells were analyzed to determine the mean fluorescence index (MFI) of PCNA expression (C) and the percentage of annexin-V-positive cells (D) , respectively. (C) Representative flow cytometry assays are shown in the left panel, and the MFI of PCNA is shown in the right panel. (D) Representative flow cytometry assays (left panel) and the frequencies (right panel) of annexin-V-positive IEC-6 cells are presented. Data corresponding to MFIs or frequencies are representatives of at least three mice in each group, presented as the mean ± SEM. Two-tailed Student’s unpaired t -tests were used for statistical analysis. *** P < 0.0001; ** P < 0.01; * P < 0.05 n.s., not significant.
    Figure Legend Snippet: (A, B) Cell permeability in murine epithelial IEC-6 cells following treatment of 5-FU. (A) After 5-FU treatment, permeability of IEC-6 cells increases in a concentration-dependent manner (5 and 10 μM). (B) siRNA-mediated knockdown of SPAK expression in IEC-6 cells blocks this increase in cell permeability compared to control cells treated with 5-FU (10 μM). (C, D) SPAK-knockdown modulates the effects of 5-FU treatment on the proliferation and survival of IEC-6 cells. Control and SPAK-KD IEC-6 cells were treated with 10 µM 5-FU and dimethyl sulfoxide (DMSO) solvent for 3 h, respectively. Untreated and 5-FU-treated control or SPAK-KD IEC-6 cells were analyzed to determine the mean fluorescence index (MFI) of PCNA expression (C) and the percentage of annexin-V-positive cells (D) , respectively. (C) Representative flow cytometry assays are shown in the left panel, and the MFI of PCNA is shown in the right panel. (D) Representative flow cytometry assays (left panel) and the frequencies (right panel) of annexin-V-positive IEC-6 cells are presented. Data corresponding to MFIs or frequencies are representatives of at least three mice in each group, presented as the mean ± SEM. Two-tailed Student’s unpaired t -tests were used for statistical analysis. *** P < 0.0001; ** P < 0.01; * P < 0.05 n.s., not significant.

    Techniques Used: Permeability, Concentration Assay, Knockdown, Expressing, Control, Solvent, Fluorescence, Flow Cytometry, Two Tailed Test



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    (A, B) Cell permeability in murine epithelial <t>IEC-6</t> cells following treatment of 5-FU. (A) After 5-FU treatment, permeability of IEC-6 cells increases in a concentration-dependent manner (5 and 10 μM). (B) siRNA-mediated knockdown of SPAK expression in IEC-6 cells blocks this increase in cell permeability compared to control cells treated with 5-FU (10 μM). (C, D) SPAK-knockdown modulates the effects of 5-FU treatment on the proliferation and survival of IEC-6 cells. Control and SPAK-KD IEC-6 cells were treated with 10 µM 5-FU and dimethyl sulfoxide (DMSO) solvent for 3 h, respectively. Untreated and 5-FU-treated control or SPAK-KD IEC-6 cells were analyzed to determine the mean fluorescence index (MFI) of PCNA expression (C) and the percentage of annexin-V-positive cells (D) , respectively. (C) Representative flow cytometry assays are shown in the left panel, and the MFI of PCNA is shown in the right panel. (D) Representative flow cytometry assays (left panel) and the frequencies (right panel) of annexin-V-positive IEC-6 cells are presented. Data corresponding to MFIs or frequencies are representatives of at least three mice in each group, presented as the mean ± SEM. Two-tailed Student’s unpaired t -tests were used for statistical analysis. *** P < 0.0001; ** P < 0.01; * P < 0.05 n.s., not significant.
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    (A, B) Cell permeability in murine epithelial IEC-6 cells following treatment of 5-FU. (A) After 5-FU treatment, permeability of IEC-6 cells increases in a concentration-dependent manner (5 and 10 μM). (B) siRNA-mediated knockdown of SPAK expression in IEC-6 cells blocks this increase in cell permeability compared to control cells treated with 5-FU (10 μM). (C, D) SPAK-knockdown modulates the effects of 5-FU treatment on the proliferation and survival of IEC-6 cells. Control and SPAK-KD IEC-6 cells were treated with 10 µM 5-FU and dimethyl sulfoxide (DMSO) solvent for 3 h, respectively. Untreated and 5-FU-treated control or SPAK-KD IEC-6 cells were analyzed to determine the mean fluorescence index (MFI) of PCNA expression (C) and the percentage of annexin-V-positive cells (D) , respectively. (C) Representative flow cytometry assays are shown in the left panel, and the MFI of PCNA is shown in the right panel. (D) Representative flow cytometry assays (left panel) and the frequencies (right panel) of annexin-V-positive IEC-6 cells are presented. Data corresponding to MFIs or frequencies are representatives of at least three mice in each group, presented as the mean ± SEM. Two-tailed Student’s unpaired t -tests were used for statistical analysis. *** P < 0.0001; ** P < 0.01; * P < 0.05 n.s., not significant.

    Journal: Frontiers in Oncology

    Article Title: SPAK Deficiency Attenuates Chemotherapy-Induced Intestinal Mucositis

    doi: 10.3389/fonc.2021.733555

    Figure Lengend Snippet: (A, B) Cell permeability in murine epithelial IEC-6 cells following treatment of 5-FU. (A) After 5-FU treatment, permeability of IEC-6 cells increases in a concentration-dependent manner (5 and 10 μM). (B) siRNA-mediated knockdown of SPAK expression in IEC-6 cells blocks this increase in cell permeability compared to control cells treated with 5-FU (10 μM). (C, D) SPAK-knockdown modulates the effects of 5-FU treatment on the proliferation and survival of IEC-6 cells. Control and SPAK-KD IEC-6 cells were treated with 10 µM 5-FU and dimethyl sulfoxide (DMSO) solvent for 3 h, respectively. Untreated and 5-FU-treated control or SPAK-KD IEC-6 cells were analyzed to determine the mean fluorescence index (MFI) of PCNA expression (C) and the percentage of annexin-V-positive cells (D) , respectively. (C) Representative flow cytometry assays are shown in the left panel, and the MFI of PCNA is shown in the right panel. (D) Representative flow cytometry assays (left panel) and the frequencies (right panel) of annexin-V-positive IEC-6 cells are presented. Data corresponding to MFIs or frequencies are representatives of at least three mice in each group, presented as the mean ± SEM. Two-tailed Student’s unpaired t -tests were used for statistical analysis. *** P < 0.0001; ** P < 0.01; * P < 0.05 n.s., not significant.

    Article Snippet: Murine intestinal epithelial IEC-6 cells (Bioresource Collection and Research Center, Hsinchu, Taiwan) were cultured in Dulbecco’s modified Eagle’s medium containing 5% fetal bovine serum, 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, and 10 mM sodium pyruvate supplemented with 0.1 U/mL bovine insulin, as described previously ( ).

    Techniques: Permeability, Concentration Assay, Knockdown, Expressing, Control, Solvent, Fluorescence, Flow Cytometry, Two Tailed Test